Current approaches for genetic engineering of plants via the VirE2-dependent pathway by A. tumefaciens is very inefficient, often results in undesirable gene duplications and chromosomal rearrangements, and does not allow for gene targeting by homologous recombination. This technology describes using GALLS-dependent transformation by Agrobacterium as a novel pathway for genetic transformation of plants that has the potential to overcome some of the limitations of the VirE2-dependent pathway.
Features & Benefits
Background of Invention
Genetic manipulation of plants has resulted in great progress towards understanding plant biology and improvement of crops. Current plant transformation methods, however, have serious limitations. Unintended gene duplications, genomic rearrangements, and the absence of efficient gene targeting complicate introduction of desirable transgenes. To overcome these limitations, it is crucial to gain a greater understanding of the mechanisms of genetic transfer to plants. Accordingly, in spite of the advances in the field in developing techniques for genetic modification of plants, a need remains for the efficient transformation of desired plant species that avoids deleterious effects associated with gene duplications, multiple insertion events, and chromosomal rearrangements. The present disclosure addresses these needs and provides additional related benefits.
Patent pending and available for exclusive licensing.