Small molecule compounds and methods for culturing ES cells that provide a means to maintain pluripotency without the use of costly leukemia inhibitory factor (LIF). Depending on the compounds used, the ES cells may remain pluripotent or ultimately be driven to an ectodermal or mesodermal fate. Pluripotency or differentiated state may be confirmed by using standard cell state biomarkers.
Features & Benefits
Background of Invention
Embryonic stem (ES) cells are pluripotent cells having the ability to self-renew and generate many types of specialized cells. ES cells are commonly used to improve detection of toxic compounds during development and testing of new drugs. They also carry great promise for regenerative medicine and cell based therapies; however, limited options for maintaining the pluripotency of ES cells or driving them to a desired differentiated state hamper discovery and development.
Patent pending; available for exclusive licensing